Figure 5From: Interferon regulatory factor 3 plays an anti-inflammatory role in microglia by activating the PI3K/Akt pathway Ad-IRF3 activates pAkt in human microglia. (A) Microglial cultures were infected (or not) with Ad-GFP or Ad-IRF3 for 48 h, then analyzed by SDS-PAGE and western blotting for p-Akt (Ser 473), p-Erk, p-Jnk and total Akt as described in the Methods section. (B) Densitometry was performed with ImageJ and the ratios of pAkt to total Akt were calculated from 3 different microglial cases. * p < 0.05 by t-test (GFP vs. IRF3).Back to article page