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Figure 6 | Journal of Neuroinflammation

Figure 6

From: Interferon regulatory factor 3 plays an anti-inflammatory role in microglia by activating the PI3K/Akt pathway

Figure 6

The effect of the PI3K/Akt inhibitor on Ad-IRF3-mediated changes in microglial gene expression. Microglial cells were left uninfected (Ctr) or infected with Ad-GFP or Ad-IRF3 for 48 h. The cells were then pre-treated with LY294002 (10 μM) for 1 h followed by stimulation with IL-1β and IFNγ for an additional 16 h. (A) Microarray analysis was performed using the Illumina platform, and the data are presented as % change (Ad-IRF3 over Ad-GFP) as described in the Results section. The data show that LY294002 reversed the effect of Ad-IRF3 in microglial inflammatory gene expression, showing suppression of IRF3-enhanced genes and enhancement of IRF3-suppressed genes. (B, C) Q-PCR confirmation of the microarray data (B: IRF3-enhanced genes, C: IRF3-suppressed genes). Q-PCR analysis was performed using PBDA as an internal control as described in Methods. At least 3 different cases were tested in each experiment. All data were normalized to the level in Ad-GFP culture (= 1).

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