Figure 8

The effect of LY294002 on PIC-, IL-1/IFNγ-, or Ad-IRF3-induced microglial cytokine expression (ELISA). Microglia cultures were grown in 96-well plates, infected (or not) with Ad-GFP or Ad-IRF3 for 48 h. Cultures were then pretreated with LY (10 μM) for 1 h before stimulation with PIC (10 μg/ml) or IL-1β/IFNγ (10 ng/ml each) for an additional 24 h. Culture supernatants or cell lysates were analyzed for IFNβ (A), IL-8 (B) and IL-1β (C) by ELISA as described in the Methods section. All samples were tested in triplicates, and p-values was determined by t-test (with vs. without LY294002). * p < 0.05, ** p < 0.01.