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Figure 9 | Journal of Neuroinflammation

Figure 9

From: Interferon regulatory factor 3 plays an anti-inflammatory role in microglia by activating the PI3K/Akt pathway

Figure 9

The effect of the PI3K pathway inhibitor LY294002 on astrocyte cytokine and chemokine expression. Primary human fetal astrocytes were stimulated with IL-1/IFNγ or PIC, with or without LY294002, essentially in the same manner described for microglia. Q-PCR (A) or ELISA (B) was performed to determine the expression of "proinflammatory" (IL-8, TNFα, and CXCL1) genes or IFNβ gene. (C) TaqMan Q-PCR was performed to determine the expression of microRNA, miR-155. All values were normalized to those without LY294002 and t-test was used to determine p values. * p < 0.05, ** p < 0.01, *** p < 0.001.

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