Effects of an IGF-I receptor antagonist on uninfected astrocytes and on astrocytes incubated with the adenoviral vectors. mRNA levels of IL6 (A,B), IL-1β (C,D), TNF-α (E,F) and TLR4 (G,H) in astrocytes 24 h after the addition of RAd-TK/GFP or RAd-IGF-I and treated for the last 5 h with the IGF-I receptor tyrosine kinase inhibitor PPP or its vehicle in the absence (A,C,E,G) or the presence of LPS (B,D,F,H). Data are presented as mean ± SEM and are expressed as percentage of control (uninfected cells treated with the vehicle for PPP) values. *,**,***, Significant differences (* P < 0.05; **P < 0.01; ***P < 0.001) in comparison to control values. +,++,+++, Significant differences between PPP and its vehicle.