Figure 1

Immunostaining of COX-1 and cell type-specific proteins in the mouse brain after ICH. A: COX-1 was constitutively expressed in the contralateral striatum (CL) and did not change significantly from 5 h to 1 day after ICH. The insets in A are representative COX-1-expressing cells at higher magnification (Scale bar, 10 μm). B-D: Double immunostaining of COX-1 with cell type-specific markers for neurons (NeuN), microglia (CD11b), and astrocytes (GFAP) in the striatum surrounding the hematoma at 3 days after ICH. Insets in B and C show a double-stained cell at higher magnification (Scale bar, 10 μm). Scale bar in the middle panels of B, C, D, 30 μm. E: Quantification analysis confirmed that the number of COX-1-immunoreactive neurons and microglia did not change in the perihematomal region of the striatum from 5 h to 3 days after ICH (n = 3/group, all P > 0.05). Values are means ± S.D.