Figure 4

Immunostaining of mPGES-1 and cell type-specific proteins (NeuN for neurons, CD11b for microglia, and GFAP for astrocytes) in the mouse brain after ICH. (A) Minimal immunoreactivity of mPGES-1 was detected in neuron-like cells in the contralateral striatum (CL). B-D: In the perihematomal region of the striatum, mPGES-1 immunoreactivity was undetectable at 5 h (B) but began to increase in glia-like cells at 1 day (C). It was additionally increased in glia-like cells at 3 days (D). Insets in A, C, and D are representative mPGES-1-expressing cells at higher magnification (Scale bar, 15 μm). E-F: Double immunostaining showed induction of mPGES-1 in neurons (E), but not in microglia (F), in the ipsilateral frontal-parietal cortex at 1 day. G-H: In the perihematomal region of the striatum, double immunostaining showed that mPGES-1 was induced primarily in astrocytes at 1 day (G); the number of mPGES-1-immunoreactive astrocytes was additionally increased at 3 days (H). Scale bar in D-H, 30 μm. Insets in E, G, and H are double-stained cells at higher magnification (Scale bar, 15 μm). Quantification analysis (I) confirmed that the number of mPGES-1 immunoreactive cells increased at 1 day, and further increased at 3 days in the perihematomal region of the striatum (n = 3/group, * P < 0.05, ** P < 0.01 compared with the CL, ^# P < 0.01 compared with day 1). Values are means ± S.D.