Figure 5

Immunostaining of mPGES-2 and cell type-specific proteins (NeuN for neurons, CD11b for microglia, and GFAP for astrocytes) in the mouse brain after ICH. A: Immunoreactive staining for mPGES-2 was observed in neuron-like cells in the contralateral striatum (CL). B-D: In the perihematomal region of the striatum, mPGES-2 immunoreactivity did not change significantly between 5 h (B) and 1 day (C) or 3 days (D) post-ICH. E-G: In the perihematomal region of the striatum, double immunostaining revealed that mPGES-2 was present in neurons at 1 day (E), but not in microglia at 1 day (F) or astrocytes at 3 days (G). Scale bar in D-G, 30 μm. Inset in E is a double-stained cell at higher magnification (Scale bar, 20 μm). Quantification analysis (H) confirmed that the number of mPGES-2-immunoreactive cells did not change in the perihematomal region of the striatum from 5 h to 3 days after ICH (n = 3/group, all P > 0.05). Values are means ± S.D.