Brain-sequestered cell numbers in C57Bl/6 mice of control mice (n = 6) and of mice infected with DENV-3. C57Bl/6 mice were infected with 4 × 103 PFU of DENV-3 by the intracranial route, and assessed at days 3 (n = 4) and 6 p.i. (n = 4). Brain-sequestered cells were counted and then stained with specific antibodies. Flow cytometry, with assessment according to size and granularity, was performed as analysis. Numbers of neutrophils (A), and CD4+ (B) and CD8+ (C) T lymphocytes were evaluated in WT mice. Results are expressed as mean ± SEM, and *p < 0.05, ***p < 0.001 when compared to non-infected mice. ###p < 0.001 when compared to 3 p.i. infected mice.