TNF-α induces P115RhoGEF phosphorylation in Bend.3 cells. Serum-starved cells were labeled with 32P for 4 h in phosphate-free medium. Bend.3 cells were then stimulated with TNF-α for the indicated times and p115RhoGEF phosphorylation was detected by autoradiography followed by western blotting. Untreated Bend.3 cells served as controls. TNF-α induced rapid p115RhoGEF phosphorylation within 1 min. (n = 3 independent experiments). *P < 0.05 vs. Control. -, absence; +, presence.