Table 1 The sequences of primers and probes used to amplify cytokine cDNA
Cytokine | 5' primer | 3' primer | Probe |
|---|---|---|---|
GDNF | GCCACCATCAAAAGACTGAAAAG | CGGTTCCTCTCTCTTCGAGGA | TCACCAGATAAACAAGCGGCGGCA |
IFN-γ | TCGAATCGCACCTGATCACTA | GGGTTGTTCACCTCGAACTTG | CATCCTTTTTTGCTTTACTGTTGCTGAGAAG |
IL-1β | GAAAGACGGCACACCCACC | AAACCGCTTTTCCATCTTCTTCT | TGCAGCTGGAGAGTGTGGATCCCAAAC |
IL-10 | CCCTCTGGATACAGCTGCG | GCTCCACTGCCTTGCTTTTATT | CGCTGTCATCGATTTCTCCCCTGTGA |
MMP-9 | GTATGGTCGTGGCTCTAAACCTG | TCGGCTGTGGTTCAGCTG | CCCAAGGCCTCCAGCCACCAC |
TGF-β1 | TGAGTGGCTGTCTTTTGACGTC | CCTGTATTCCGTCTCCTTGGTT | CTGGAGTTGTCCGGCAGTGGCTGA |
TNF-α | GACCCTCACACTCAGATCATCTTCT | ACGCTGGCTCAGCCACTC | TAGCCCACGTCGTAGCAAACCACCAA |