Figure 4

Co-localization of A _2A receptors with astrocytes (GFAP) in white matter of SC 24 h after injury. Double immunofluorescence was used to characterize the co-localization of A_2A receptors (in red with Texas red) with astrocytes (in green with fluorescein) in sham-operated and SCI mice groups. The merged images show that A_2A receptors are present on astrocytes both before and after SCI. The co-localization is quite total. The drawing under figures shows that A_2A receptors were identified in the white matter area as indicated by arrow. Scale bar = 10 μm.