Effect of systemic SCH58261-administration on JNK MAPK activation. Western blot analysis shows a significant increase in phospho-JNK MAPK (Thr183/Tyr185) 24 h after SCI. SCH58261, intraperitoneally administered three times within 24 h, reduced SCI-induced phospho-JNK MAPK levels. A representative blot of lysates obtained from each mice group is shown. The densitometric analysis is expressed as the mean ± SEM for samples from 10 mice from each group and is normalized by control protein (β-actin) levels, and reported in a bar graph. One-way ANOVA: *P < 0.01 vs sham; ##P < 0.05 vs SCI.