Figure 2

HIF-1α and NF-kB expression in host, peritumor and tumor fractions obtained by neuronavigation. (A) Host (H), peritumor (P) and tumor (T) tissues of GBM were obtained by neuronavigation. The content of HIF-1α was determined by western blotting. Values obtained from densitometric analyses of cytosolic and nuclear fractions from all samples analyzed (10) are shown. β-Actin and Lamin A/C were used as loading controls for the cytosolic and nuclear fractions, respectively. Data are presented as mean ± SD for all GBMs analyzed (n = 10). *, P < 0.05.(B) Left side: Host (H), peritumor (P) and tumor (T) tissue of GBM were obtained by neuronavigation. HIF-1α content was measured in the nuclear fractions by Trans-AM ELISA assay as described in Methods. Right side: VEGF, EPO and GLUT1 mRNA expression was measured in H, P and T tissues by RT-PCR. Data are presented as mean ± SD for all GBMs analyzed (n = 10). *, P < 0.05.(C) Host (H), peritumor (P) and tumor (T) tissues of GBM were obtained by neuronavigation. Content of the NF-kB subunit p65 was determined by western blotting. Values obtained from densitometric analysis of cytosolic and nuclear fractions from all samples analyzed (n = 10) are shown. β-Actin and Lamin A/C were used as loading controls for the cytosolic and nuclear fractions, respectively. Data are presented as mean ± SD for all GBMs analyzed (n = 10). *, P < 0.05.