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Figure 2 | Journal of Neuroinflammation

Figure 2

From: The CCAAT/enhancer binding protein (C/EBP) δ is differently regulated by fibrillar and oligomeric forms of the Alzheimer amyloid-β peptide

Figure 2

Fibril-enriched Aβ inhibits IL-1β-induced C/EBPδ expression in rat primary astro-microglial cultures. A) Representative western blot of C/EBPα (p42 and p30) and C/EBPδ in primary astro-microglial cultures from newborn rat. Cells were exposed to an Aβ preparation highly enriched in fibrils for 3 h. 20 μg of protein lysate was loaded in each lane. B) Relative abundance of C/EBPα showing significantly decreased protein levels in response to LPS (1 μg/ml), but no significant effect in response to fibril-enriched Aβ (10 μM) or IL-1β (10 ng/ml). C) Relative abundance of C/EBPδ showing increased levels in response to IL-1β or LPS, and decreased C/EBPδ levels after exposure to IL-1β together with Aβ compared to treatment with IL-1β alone. The data represent mean ± SEM for two cultures derived from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, significantly different from control or otherwise indicated.

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