Figure 2
Proinflammatory mediator production following CNS C. koseri infection is influenced by both MyD88-dependent and -independent signals. MyD88 WT and KO mice (4-6 per group) received intracerebral injections of C. koseri (3.4 × 104 cfu), whereupon supernatants were prepared from infected hemispheres and cytokine/chemokine expression evaluated using multiplex microbead arrays for IL-1β (A), TNF-α (B), IL-6 (C), CXCL1 (D), CXCL2 (E), CXCL10 (F), CCL2 (G), and CCL5 (H). Cytokine/chemokine expression was normalized based on the amount of total protein obtained for each sample to correct for differences in tissue sampling size. Significant differences in mediator expression between MyD88 WT and KO mice at 12 h post-infection are denoted by asterisks (*, p < 0.05, **, p < 0.01, t-test), whereas significant differences in mediator levels between MyD88 WT and KO animals at 24 h post-infection are denoted by hatched signs (#, p < 0.05, ##, p < 0.01, t-test). Results are representative of two independent experiments with a total of 3-6 mice/group/study.