Figure 6
TLR4 has minimal impact on the course of CNS C. koseri infection. TLR4 WT and TLR4 mutant mice (triangles and circles, respectively; 4-5 per group) received intracerebral injections of C. koseri (2.8 × 104 cfu) to induce meningitis and brain abscess formation. Survival rates of WT and TLR4 mutant mice were 100% and 84%, respectively (A). TLR4 WT or TLR4 mutant (Mut) mice were euthanized at days 3 or 7 post injection (4-5 mice per group), whereupon the number of viable bacteria in either the injected (B) or contralateral (C) hemispheres were determined. Bacterial burdens are expressed as colony forming units (cfu) of C. koseri per gram of wet tissue weight (each data point represents an individual animal). Results are representative of three independent experiments with a total of 4-6 mice/group/study.