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Figure 7 | Journal of Neuroinflammation

Figure 7

From: MyD88 is pivotal for immune recognition of Citrobacter koseri and astrocyte activation during CNS infection

Figure 7

Proinflammatory mediator production in response to CNS C. koseri infection is primarily TLR4-independent. TLR4 WT and TLR4 mutant mice (4-5 per group) received intracerebral injections of C. koseri (2.8 × 104 cfu), whereupon supernatants were prepared from infected hemispheres and cytokine/chemokine expression evaluated using multiplex microbead arrays for CCL2 (A), CXCL1 (B), IL-1β (C), and CXCL10 (D). Cytokine/chemokine expression was normalized based on the amount of total protein obtained for each sample to correct for differences in tissue sampling size. Significant differences in mediator expression between days 3 and 7 post-infection are denoted by asterisks (*, p < 0.05, One-Way ANOVA), whereas the difference in CXCL10 levels between TLR4 WT mice and TLR4 mutant mice at day 3 post-infection is denoted by a hatched sign (#, p < 0.05, One-Way ANOVA). Results are representative of three independent experiments with a total of 4-6 mice/group/study.

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