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Figure 9 | Journal of Neuroinflammation

Figure 9

From: MyD88 is pivotal for immune recognition of Citrobacter koseri and astrocyte activation during CNS infection

Figure 9

Astrocyte activation in response to heat-inactivated or viable C. koseri is TLR4-independent. FACS-enriched WT and TLR4 mutant astrocytes were plated at 1 × 105 cells/well in 96-well plates and exposed to either heat-inactivated C. koseri (107 cfu/well) for 24 h (A, B, C), or live C. koseri at a multiplicity of infection (MOI) of 1 for 6 h (D, E, F). CCL2 (A and D) and CXCL2 (B and E) production was quantitated by ELISA. Astrocyte viability was assessed using a MTT assay and the raw OD570 absorbance values are reported (C, F). There were no significant differences in chemokine release between WT and TLR4 mutant astrocytes. Results are reported as the mean ± SD of three independent wells for each experimental treatment and were identical across six separate experiments.

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