Figure 2

Effects of PKC inhibitors on iNOS expression in BV-2 cells. A, BV-2 cells were treated with LPS (1 μg/ml) for 6 hr in the presence of PKC inhibitors at the indicated concentrations. Aliquots of cell extracts prepared from the above treated cultures were subjected to western blot analysis by using antibodies against iNOS and β-actin. A representative experiment of four that were performed is shown. B, The levels of iNOS expression with various treatment conditions were normalized by β-actin and quantified using densitometric analysis. The data were pooled from 4 different experiments. **, p < 0.01 and ***, p < 0.001 were obtained when the GO, RO and BIS treated groups were compared to the LPS alone group. C. At 24 hr following LPS (1 μg/ml) treatment in the absence or presence of PKC inhibitors, culture media from 24-well plates were collected for measurement of nitrite production by reactive microglia. **, p < 0.01 and ***, p < 0.001 were obtained when the GO-, RO- and BIS-treated groups were compared to the LPS alone group. A representative experiment of four that were performed is shown.