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Figure 3 | Journal of Neuroinflammation

Figure 3

From: Specific PKC isoforms regulate LPS-stimulated iNOS induction in murine microglial cells

Figure 3

Time course of MAPK activation induced by LPS in BV-2 cells. BV-2 cells were treated with LPS (1 μg/ml) for 5, 15, 30 min, 1 or 2 hr and whole cell lysates at different time points were collected and subjected to western blot with antibodies against phosphorylated and total p38, JNK and ERK1/2, respectively. Phosphorylation of p38 occurred at 5 min following LPS treatment, whereas phosphorylation of ERK1/2 and JNK was observed after 15 min of LPS treatment. The phosphorylation of all three MAPKs started to diminish after 1 hr of LPS treatment. A representative experiment of three that were performed is shown.

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