Figure 7

Effects of downregulation of PKC isoforms on iNOS expression in LPS-activated BV-2 cells. A, At 48 hr following nPKC siRNA transfection, cells were treated with LPS (1 μg/ml) for 6 hr and then lysed for western blot analysis. The ratio of relative band intensity of iNOS to β-actin from 4 different experiments is shown. *, p < 0.05 and ***, p < 0.001 were obtained when the PKC η, θ and δ siRNA transfected groups were compared to the RISC-free siRNA control group. B, At 48 hr following cPKC siRNA transfection, cells were treated with LPS (1 μg/ml) for 6 hr and then lysed for western blot analysis. The ratio of relative band intensity of iNOS to β-actin from 3 different experiments is shown. ***, p < 0.001 was obtained when the PKC β siRNA transfected group was compared to the RISC-free siRNA control group.