Figure 8

Effects of downregulation of PKC isoforms on the phosphorylation of MAPK in LPS-treated BV-2 cells. A, At 48 hr following PKC δ, η, ε or θ siRNA transfection, cells were treated with LPS (1 μg/ml) for 15 min and then lysed for western blot using antibodies against phosphorylated and total ERK1/2, p38 and JNK. A representative experiment of 3 that were performed is shown. B, At 48 hr following PKC α or β siRNA transfection, cells were treated with LPS (1 μg/ml) for 15 min and then lysed for western blot using antibodies against phosphorylated and total ERK1/2, p38 and JNK. *, p < 0.05 and **, p < 0.01 were obtained when the PKC siRNA transfection groups were compared to the RISC-free siRNA control group. The ratio of relative band intensity of phosphorylated ERK1/2 to total ERK1/2 and phosphorylated p38 to total p38 from 3 different experiments is shown.