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Figure 3 | Journal of Neuroinflammation

Figure 3

From: Overweight worsens apoptosis, neuroinflammation and blood-brain barrier damage after hypoxic ischemia in neonatal brain through JNK hyperactivation

Figure 3

Apoptosis, activated microglia and BBB damage after HI. Twenty-four hours post-HI, western blots showed that the OF-HI pups had significantly increased levels of cleaved caspase-3 (A) and PARP (B) in the cortex compared with the NF-HI pups. The OF-HI pups also had significant increases of 150kD and 120kD a-spectrin fragments than the NF-HI pups (C). n = 4-5 in each group at each time point. Immunohistochemistry showed that the OF-HI pups had significant increases of ED1-(+) activated microglia (D), and more extravasation of IgG (E) indicating BBB leakage 24 hours post-HI compared to the NF-HI pups. The representative pictures in D and E were from the ipsilateral cortex of plate 31 of the rat-brain atlas [17]. Scale bar = 100 μm in low magnifications (D, E), and 20 μm in high magnifications (D). *p < 0.05, **p < 0.01, Data are means ± SEM.

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