Figure 5
JNK hyperactivation in the NF and OF pups. (A) Western blots showed significantly higher p-JNK but not Grp78 levels in the OF pups compared to the NF pups on P7. After HI, the OF-HI pups also had significantly higher p-JNK (B) but not Grp78 or phospho-p38 (p-p38) (C) levels immediately post-HI than the NF-HI pups. (D) In vitro kinase assay of JNK showed significant increases of p-GST-c-Jun levels one hour post-HI in the OF-HI pups. (E) The OF pups had higher levels of p-BimEL instead of p-c-Jun immediately post-HI than the NF pups. n = 4~5 in each time point of each group, *p < 0.05, Data are means ± SEM.