Figure 7
JNK inhibition against HI in the NF and OF pups. (A) Intracerebroventricular injections of AS601245 (AS, 100-nmol, 150-nmol) effectively diminished JNK activity one hour post-HI in the OF-HI and NF-HI pups. AS injections decreased p-BimEL levels but did not affect p-JNK levels in the OF-HI pups. n = 4 experiments. (B) Compared with their respective vehicle-treated pups, JNK inhibition (150-nmol AS) caused more reduction of cleaved levels of caspase-3 and PARP, and spectrin fragments in the OF-HI pups than in the NF-HI pups. AS 150-nmol treatment significantly decreased ED1-(+) microglia (C) and IgG extravasation (D) in the cortex 24 hours post-HI in the OF-HI but not in the NF-HI pups. n = 4-5 experiments. The representative pictures were from the ipsilateral cortex in plate 31 of the rat-brain atlas [17]. (E) Brain-volume loss measured on P21 showed the more protective effects of AS in the OF-HI compared to the NF-HI pups. Scale bar = 100 μm. *p < 0.05, **p < 0.01. Data are means ± SEM.