Figure 1

α-synuclein transfected BV2 cells increased Cox-2 protein expression. BV2 cells were transiently transfected to express WT, A30P, or A53T α-synuclein for 48 hours. A) Cells were lysed and Western blotted using anti-cPLA₂, anti-Cox-1, anti-Cox-2, anti-PLD1, anti-PLD2, anti- α-synuclein, or anti-GAPDH (loading control) antibodies. Protein levels of B) cPLA₂, C) Cox-1, D) Cox-2, E) PLD1, and F) PLD2 were quantified and normalized to GAPDH. Graphs are the average (± SD) of five independent experiments. * p < 0.05 compared to mock transfected cells.