Figure 2
From: Expression of mutant alpha-synuclein modulates microglial phenotype in vitro
α-synuclein over-expression was not toxic to BV2 cells. BV2 cells were transiently transfected to express WT, A30P, or A53T α-synuclein for 48 hours. An LDH release assay was performed to determine cell viability by quantifying LDH release into the medium using commercial LDH assay reagents. Graphs are the average (± SD) of three independent experiments. Each experiment was performed with 8 replicates per condition.