Over-expression of α-synuclein increased TNF-α, IL-6, and nitrite levels in medium from BV2 cells. BV2 cells were transiently transfected to express WT, A30P, or A53T alpha-synuclein for 48 hours with or without 25 ng/ml LPS stimulation. Media were collected and used for quantifying concentrations of secreted A) and B) TNF-α, C) IL-6 using a commercial mouse TNF-α and IL-6 ELISA. D) Media was also used to perform Griess assay to detect the levels of nitrite secreted from BV2 cells. Each condition was performed with 8 replicates. Graphs are the average (± SD) of three independent experiments. ** p < 0.01, *** p < 0.001.