Over-expression of α-synuclein did not increase neurotoxic secretion from BV2 cells. BV2 cells were transiently transfected to express WT, A30P, or A53T α-synuclein then co-cultured onto a membrane insert with 7 days in vitro mouse cortical neurons for 72 hours in the A) absence or B) presence of 25 ng/ml LPS stimulation. After 72 hours, the inserts were removed and the neurons were fixed in 4% paraformaldehyde and immunostained with an anti-MAP2 antibody. MAP2 positive cells were counted to assess viability. Experiments were performed with 8 replicates per condition. Graphs are the average (± SD) of three independent experiments. *** p < 0.001 compared to neurons only.