Figure 3
From: Regulation of Toll-like receptor 1 and -2 in neonatal mice brains after hypoxia-ischemia
Neurons express TLR-1 24 h after hypoxia-ischemia. A: Triple immunofluorescence staining for TLR-1, NeuN and Iba-1; the merged picture displays expression of TLR-1 by NeuN positive neurons but not Iba-1 positive microglia. B: Triple immunofluorescence staining for TLR-1, GFAP and Olig2; The merged picture shows mutually exclusive expression of TLR-1, GFAP Olig2 and no co-localisation of all three markers. C: High magnification orthogonal views of cells stained with TLR-1 and NeuN; the merged picture shows membranous localisation of TLR-1 surrounding NeuN positive nuclei. All images were captured in the ipsilateral hippocampus. Scale bars: A-B: 25 μm C: 10 μm