Caspase 8 mediates CoMTb induced MMP-2 suppression. (A) Caspase 8 activity is up-regulated by CoMTb at 30 minutes and returned to baseline by the inhibitor z-IETD-fmk. Representative Western blot shown. (B) Caspase 8 inhibition restores CoMTb-mediated MMP-2 suppression. 72 h supernatants were analyzed by Luminex and confirmed by gelatin zymography. Bars represent mean values ± SD of three samples, representative of at least duplicate experiments performed in triplicate. Data were analyzed by one-way analysis of variance, followed by Tukey's multiple comparison. **p < 0.01. (C) Phospho-p38 activity at 30 minutes is up-regulated by caspase 8 inhibition. Representative Western blot shown. Total p38 blot to show equal lane loading. (D) Caspase 8 inhibition does not alter NFκB nuclear dynamics. Nuclear extracts of CoM stimulated cells were taken at 30 mins and p50 & p65 subunits measured by ELISA.