Axl-/- mice have a more severe clinical course than WT mice during MOG-induced EAE. a) EAE clinical course for Axl-/- and WT female mice. Three experiments were combined: WT (n = 15) and Axl-/- (n = 21) mice. All mice were housed in Barrier. b) Western blot analysis was performed using an Axl pAb on 80 μg of WT naïve (untreated), WT EAE, Tyro3-/- naïve (untreated), and Tyro3-/- EAE lumbar spinal cord homogenates. The mature Axl band migrates at 140 kDa, and the immature band (lacking post translational modifications) migrates at approximately 110 kDa. N = 3 for all groups except WT EAE where n = 4. c) The relative densitometric intensity (rdi) was determined for each band and normalized to β-actin. The average values for 140 kDa Axl are shown. * = p < 0.05, # = p < 0.01.