Figure 5

Demyelination and infiltration of CD45+ cells in spinal cords of Axl-/- mice are enhanced relative to WT mice. Seven micron paraffin-embedded day 14 WT and Axl-/- spinal cord sections were stained with MBP or CD45 mAb's, and frozen sections were stained with an Axl pAb, and were visualized by DAB. Representative images of WT (a,b) and Axl-/- (g,h,i,j,k) spinal cord stained for MBP. Arrows in a,g,i denote areas of demyelination shown at 40× in b,h,j, respectively. Asterisks in i denote demyelinated lesions; higher magnification of one lesion at 40× is shown in k. e,f) images of a WT mouse stained for Axl; the boxed area in e is shown at 20× in f. c,l) low magnification images of WT (c) and Axl-/- (l) mice stained with CD45. d) serial section of b stained for CD45. Serial sections of j,k stained for CD45 are shown in m,n.