CBD inhibited leukocyte margination. (A) Representative 60 μm Z-stacks of subpial vessels at t = 0 (left) and t = 120 min (right) after LPS administration. Note the intense accumulation of nucleated cells in the luminal side of blood vessels at t = 120 min, detectable as static black dots (arrows). (B) Quantification of CBD effect on cell margination (expressed as immobilized cells/μm2), reflecting a dramatic inhibition of the process by this cannabinoid over the experimental time course. Vehicle (open circles), LPS (black circles) and LPS+CBD (triangles). Error bars represent mean ± SEM of 7-8 experiments. †p < 0.05 by 2-way ANOVA. Scale bar: 250 μm.