Figure 4From: Transcellular migration of neutrophil granulocytes through the blood-cerebrospinal fluid barrier after infection with Streptococcus suis S. suis and TNFα alter the morphology of the tight junction protein occludin and induce actin stress fiber formation after the transmigration of PMNs across PCPECs. (A-P) En face Apotome microscopy of control (A-D), S. suis (strain 10: E-H, strain 10 Δcps: I-L) and TNFα- (M-P) stimulated PCPEC monolayers stained for occludin (red), actin (phalloidin, green) and nuclei (DAPI, blue) after PMN (yellow) transmigration. Top and side of each panel is a cross section through the z-plane of multiple optical slices. This figure is a representative example of three independent experiments that all gave similar results. Scale bar, 10 μm.Back to article page