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Table 1 Antibodies used for inhibition studies, flow cytometry and immunofluorescence.

From: Transcellular migration of neutrophil granulocytes through the blood-cerebrospinal fluid barrier after infection with Streptococcus suis

Antibody Clone Species Concentration Company
Primary antibodies
IgG 1 , κ Isotype MOPC-31C Mouse 20 μg/ml (inhibition)
1:10, 10 μl/test (FC)
BD Pharmingen (Heidelberg, Germany)
IgG 2b , κ Isotype MPC-11 Mouse 20 μg/ml (inhibition)
1:10, 10 μl/test (FC)
BD Pharmingen (Heidelberg, Germany)
CD11a, IgG2b BL2F1 Mouse 20 μg/ml (inhibition)
1:10, 10 μl/test (FC)l
BD Pharmingen (Heidelberg, Germany)
CD11R3, IgG1
= CD11b
2F4/11 Mouse 20 μg/ml (inhibition)
1:10, 10 μl/test (FC)
Serotec (Oxford, UK)
CD18, IgG1 PNK-I Mouse 20 μg/ml (inhibition)
1:10, 10 μl/test (FC)
BD Pharmingen (Heidelberg, Germany) or Serotec (Oxford, UK)
CD47, IgG2b BRIC126 Mouse 20 μg/ml (inhibition)
1:10, 10 μl/test (FC)
Serotec (Oxford, UK)
CD49d, IgG1 HP2/1 Mouse 20 μg/ml (inhibition)
1:10, 10 μl/test (FC)
Serotec (Oxford, UK)
CD49e, IgG1 VC5 Mouse 20 μg/ml (inhibition)
1:10, 10 μl/test (FC)
BD Pharmingen (Heidelberg, Germany)
Occludin   Rabbit 1:250 (IF)
1 μg/ml
Zymed Laboratories (South San Franscisco, CA, USA)
ZO-1   Rabbit 1:250 (IF)
1 μg/ml
Zymed Laboratories (South San Franscisco, CA, USA)
SWC3a-FITC 74-22-15 Mouse 1:10 (IF)
10 μl/Test
Southern Biotech (Birmingham, AL, USA)
Secondary antibodies
Alexa fluor ® 594 goat anti chicken   Goat 1:1000 (IF) Molecular Probes (Eugene, OR, USA)
Goat anti mouse-PE   Goat 1:10, 10 μl/test (FC) Southern Biotech (Birmingham, AL, USA)
  1. SWC: swine workshop cluster