Table 1 Antibodies used for inhibition studies, flow cytometry and immunofluorescence.

IgG ₁ , κ Isotype

MOPC-31C

Mouse

20 μg/ml (inhibition)

1:10, 10 μl/test (FC)

BD Pharmingen (Heidelberg, Germany)

IgG _2b , κ Isotype

MPC-11

Mouse

20 μg/ml (inhibition)

1:10, 10 μl/test (FC)

BD Pharmingen (Heidelberg, Germany)

CD11a, IgG_2b

BL2F1

Mouse

20 μg/ml (inhibition)

1:10, 10 μl/test (FC)l

BD Pharmingen (Heidelberg, Germany)

CD11R3, IgG₁

= CD11b

2F4/11

Mouse

20 μg/ml (inhibition)

1:10, 10 μl/test (FC)

Serotec (Oxford, UK)

CD18, IgG₁

PNK-I

Mouse

20 μg/ml (inhibition)

1:10, 10 μl/test (FC)

BD Pharmingen (Heidelberg, Germany) or Serotec (Oxford, UK)

CD47, IgG_2b

BRIC126

Mouse

20 μg/ml (inhibition)

1:10, 10 μl/test (FC)

Serotec (Oxford, UK)

CD49d, IgG₁

HP2/1

Mouse

20 μg/ml (inhibition)

1:10, 10 μl/test (FC)

Serotec (Oxford, UK)

CD49e, IgG₁

VC5

Mouse

20 μg/ml (inhibition)

1:10, 10 μl/test (FC)

BD Pharmingen (Heidelberg, Germany)

Occludin

Rabbit

1:250 (IF)

1 μg/ml

Zymed Laboratories (South San Franscisco, CA, USA)

ZO-1

Rabbit

1:250 (IF)

1 μg/ml

Zymed Laboratories (South San Franscisco, CA, USA)

SWC3a-FITC

74-22-15

Mouse

1:10 (IF)

10 μl/Test

Southern Biotech (Birmingham, AL, USA)

Secondary antibodies

Alexa fluor ^® 594 goat anti chicken

Goat

1:1000 (IF)

Molecular Probes (Eugene, OR, USA)

Goat anti mouse-PE

Goat

1:10, 10 μl/test (FC)

Southern Biotech (Birmingham, AL, USA)