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Table 2 Primers used for quantitative real-time PCR

From: Transcellular migration of neutrophil granulocytes through the blood-cerebrospinal fluid barrier after infection with Streptococcus suis

Gene

Primer

Sequence (5' → 3')

ICAM-1

forward

CACAGGCCGCCACTAACAA

ICAM-1

reverse

GGTTCCATTGATCCAGGTCTT

VCAM-1

forward

GCACGAGCTTCCTGAGCACTT

VCAM-1

reverse

CTGTGTGACGAGGAAACAATG

β-actin

forward

TCCAGAGGCGCTCTTCCA

β-actin

reverse

CGCACTTCATGATCGAGTTGA

  1. ICAM, intracellular adhesion molecule; VCAM, vascular cell adhesion molecule