IL-6 stimulates the formation of excitatory synapses. (a) Representative photomicrographs of labeling of Syp, VGLUT1 and VGAT in cultured IL-6 and control CGCs, respectively. Scale bars, 25 μm. (b) Quantification of puncta size using automatic image analysis with the NIH ImageJ program for Syp (n = 26 and 25 for control and IL-6 group, respectively), VGLUT1 (n = 23 and 25 for control and IL-6 group, respectively) and VGAT (n = 25 and 24 for control and IL-6 group, respectively). *P < 0.05 and **P < 0.01 compared with control condition.