Figure 6

BBB leakage and brain damage. Distribution of increased albumin staining within right hemispheric gray and white matters exposed to HI, LPS or LPS+HI in P12 brains compared to control (A) correlated with injured areas (B, C). Brain injuries induced by HI and LPS+HI at P12 corresponded to infarcted areas (*) located in right carotidian territory; extent severity of infarct were maximum under LPS+HI compared to HI (B). Schematic presentation of distribution of BBB leakage at P12, including albumin extravasations, PMN and CD68+ cells infiltrations in HI and LPS+HI brains showing a topographic association between all these components of the neuroinflammatory response and brain damage (C). Brain damage at P1 was detected in both white and gray matter (cortex and basal ganglia) (D). In contrast to P12 lesion, P1 brains submitted to HI and LPS+HI presented laminar (D2, black arrowheads) or columnar (D3, black arrowheads) areas of neuronal necrosis (D2, D3, empty arrowheads), linear microhemorrhage (D3, arrows) and foci of white matter damage (D1, black arrowheads) combining spongiotic cavities (D1, empty arrowheads) and disorganization of cellular architecture (D1).