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Table 1 Antibodies and dilutions used in FACS analysis of macrophages

From: Classically and alternatively activated bone marrow derived macrophages differ in cytoskeletal functions and migration towards specific CNS cell types

Primary antibody Subtype Dilution Secondary antibody Dilution
F4/80 (Serotec, Oxford, UK) IgG 1:500 Anti-rat Alexa 488
(Invitrogen)
1:400
MAC-1 (Serotec, Oxford, UK) IgG 1:500 Anti-rat Alexa-488
(Invitrogen)
1:400
MR biotinylated (Biolegend, Uithoorn, the Netherlands) IgG 1:100 Streptavidin-Alexa 488
(Invitrogen)
1:400
LFA-1 produced in house IgG 1:250 Anti-rat Alexa 488
(Invitrogen)
1:400
CD29 (BD Pharmingen, Breda, the Netherlands) IgG 1:100 Anti-rat Alexa 488
(Invitrogen)
1:400
CD11a-FITC (eBioscience, Malden, the Netherlands) IgG 1:100