Table 1 Antibodies and dilutions used in FACS analysis of macrophages
Primary antibody | Subtype | Dilution | Secondary antibody | Dilution |
|---|---|---|---|---|
F4/80 (Serotec, Oxford, UK) | IgG | 1:500 | Anti-rat Alexa 488 (Invitrogen) | 1:400 |
MAC-1 (Serotec, Oxford, UK) | IgG | 1:500 | Anti-rat Alexa-488 (Invitrogen) | 1:400 |
MR biotinylated (Biolegend, Uithoorn, the Netherlands) | IgG | 1:100 | Streptavidin-Alexa 488 (Invitrogen) | 1:400 |
LFA-1 produced in house | IgG | 1:250 | Anti-rat Alexa 488 (Invitrogen) | 1:400 |
CD29 (BD Pharmingen, Breda, the Netherlands) | IgG | 1:100 | Anti-rat Alexa 488 (Invitrogen) | 1:400 |
CD11a-FITC (eBioscience, Malden, the Netherlands) | IgG | 1:100 | Â | Â |