Hypothermia causes time-dependent changes on CD-68 expression after stroke. (A) shows the 3 fixed fields in the striatum and the cortex in which CD-68+-cells were counted using a lattice. The reference lines are represented in red. The phagocytic cells determined by expression of CD-68 in a normothermic and hypothermic rat at 72 hours after the administration of Et-1 or Ringer's are shown in (B) and (C) respectively. The graphs below show the overall expression in the striatum (D) and the cortex (E) separately. Data are expressed as the number of cells/mm2 and are shown as the mean ± SEM. There was no binding of the second antibody in the absence of the primary antibody (data not shown). *Significantly different (p < 0.05) compared to the results at 8 hours as determined by one-way ANOVA with Dunnett post-hoc test. Furthermore, §normothermic rats (n = 4, 8, 5 and 8 at 8, 24, 72 and 168 hours respectively) were compared to sham rats (n = 4 at 8, 24 and 72 hours and n = 5 at 168 hours) and hypothermic rats (n = 5, 6, 6 and 8 rats at 8, 24, 72 and 168 hours respectively) using one-way ANOVA with Dunnett post-hoc test.