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Figure 3 | Journal of Neuroinflammation

Figure 3

From: A dual role for microglia in promoting tissue inhibitor of metalloproteinase (TIMP) expression in glial cells in response to neuroinflammatory stimuli

Figure 3

Examining the role of microglia-derived IL-1β in promoting astrocyte TIMP-1 expression. A. Determination of IL-1β in LPS-activated MG-CM. Microglial cultures were treated with LPS for 2 days, then subject to either six rounds of IL-1β immunodepletion, or mock depletion, as described in Materials and Methods, and samples analyzed for the presence of IL-1β. Note that IL-1β was detected in the LPS-activated MG-CM before, but not after the IL-1β immunodepletion, demonstrating the effectiveness of the immunodepletion strategy. B and C. The effect of removing IL-1β from LPS-activated MG-CM on astrocyte TIMP-1 production. Pure astrocyte cultures received either: LPS, microglia + LPS, LPS-activated MG-CM (mock depleted) or LPS-activated MG-CM depleted of IL-1β. Note that the presence of LPS-activated microglia or LPS-activated MG-CM strongly induced TIMP-1 expression in astrocytes, but this effect was markedly attenuated after IL-1β removal from the MG-CM. * p < 0.02.

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