Table 1 Primary Antibodies used
Antigen | Host | Manufacturer | Dilution used* |
|---|---|---|---|
Calbindin D-28 K | rabbit | Cell signaling | 1:200 (IF) |
Glial fibrillary acidic protein | mouse | Millipore | 1:5,000 (IF) |
NeuN (a neuronal maker) | mouse | Millipore | 1:1000 (IF) |
NF-κB p52-Ser865 | rabbit | Abcam | 1:200 (IH) |
NF-κB p52-Ser869 | rabbit | Abcam | 1:200 (IH) |
NF-κB p65-Ser276 | rabbit | Abcam | 1:200 (IH) |
NF-κB p65-Ser311 | rabbit | Abcam | 1:200 (IH) |
NF-κB p65-Ser468 | rabbit | Abcam | 1:200 (IH) |
NF-κB p65-Ser529 | rabbit | Abcam | 1:200 (IH) |
TNF-α | goat | R&D system | 1:500 (IH) 1:200 (IF) |
TNFp55R | rabbit | Abcam | 1:200 (IF) |
TNFp75R | Rabbit | Abcam | 1:200 (IF) |