Effect of HFD and BM on brain Sirt and FoxO protein expression. Whole brain Sirt1 levels in mice fed HFD with and without BM. Figure 5A represents western blot of Sirt1 protein levels and Figure 5B demonstrates mRNA expression. The bar graph in Figure 5A represents the densitometry scan of 110-kD protein (n = 4). Figure 5C demonstrates Sirt3 mRNA expression analyzed by qRT-PCR (n = 4), while FoxO1 and FoxO3a mRNA expression are depicted in Figures 5D and 5E, respectively (n = 4). Values represent the mean ± SE. Each sample was analyzed in duplicate. a,b,cMean values with common letters do not differ (p < 0·05).