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Table 1 Primer sequences for real-time RT-PCR

From: Momordica charantia (bitter melon) attenuates high-fat diet-associated oxidative stress and neuroinflammation

Target Gene
(GenBank Acc. No.)
Primer Sequence Amplicon size (bp)
IL-22 (AJ249492)
Forward
Reverse
5'-GTGACGACCAGAACATCCAG-3'
5'-ATCTCTCCGCTCTCTCCAAG-3'
76
IL-17rb (NM_019583)
Forward
Reverse
5'-CTGTGTGGAGGTAGTGCTATG-3'
5'-CTCAGGCGTGTATGCTCAG-3'
78
NF-κB (AK011965)
Forward
Reverse
5'-CGTCGGTGCTATTCTGTTG-3'
5'-GCTGTCTGGTAAAGGTTGTTC-3'
76
IL-16 (BC026894)
Forward
Reverse
5'-AGGAAAGCAAGATGGAGGAC-3'
5'-GAGGAGTTAGGGAAGGTTGTG-3'
116
Aif1/Iba1 (NM_019467.2)
Forward
Reverse
5'-GTCCTTGAAGCGAATGCTGG-3'
5'-CATTCTCAAGATGGCAGATC-3'
157
CD11b (NM_019467)
Forward
Reverse
5'- CAGGCATCACTTCCACATCAGC-3'
5'- CTGTCTTAACCTGCATCA-3'
174
GFAP (NM_10277)
Forward
Reverse
5'- TCTCCTCCTCCAGCGATTCAAC-3'
5'- TGTGGATTTGGAGAGAAA-3'
78
S100B (NM_009115)
Forward
Reverse
5'- CTGCTTGCCATCTGCCTGAG-3'
5'- GAGGTTGCTCATCCTTAC-3'
134
Eif3s10 (AW701127)
Forward
Reverse
5'-CTCTTCAGTCCGGTCATCTTTC-3'
5'-CAAGTCGCCGTGATGATAGG-3'
194
Hsp110 (D67017)
Forward
Reverse
5'-AATGAGAAGGGCTCTGTCAAC-3'
5'-CAGGATCACATACTATGGCAAAC-3'
96
Dnajb1 (AK002290)
Forward
Reverse
5'-AGTGGTGGTGCTAATGGTAC-3'
5'-AAAAGGTATCAAAGGGGTTTCTG-3'
103
Dnaja3 (AK004575)
Forward
Reverse
5'-AGATGGTCAGACTGTAAGGATG-3'
5'-AGAGGTCCGAGTGGATGTC-3'
113
Sirt3 (NM_022433)
Forward
Reverse
5'-CTGGATGGACAGGACAGATAAG-3'
5'-TCTTGCTGGACATAGGATGATC-3'
79
Foxo1 (AI462296)
Forward
Reverse
5'-ACGGGCTGTCTGTCTGTC-3'
5'-TAAGTGAAGTTTCTCTGTGGTTTC-3'
116
Foxo3a (BB364488)
Forward
Reverse
5'-GCCTCGGTCACACTCCAG-3'
5'-TTCACACCTGTCCACATTTCC-3'
166
GAPDH (NM_001001303)
Forward
Reverse
5'-TCAACGGCACAGTCAAGG-3'
5'-ACTCCACGACATACTCAG-3'
126