Effect of midazolam on IL-1β-induced STAT3 phosphorylation. Cultured cells were pretreated with 10 μM midazolam for 60 min, and then stimulated with 10 ng/ml IL-1β or vehicle for 60 min in the presence of midazolam. Cell extracts were analyzed by Western blotting using antibodies against phospho-specific STAT3 or STAT3. The histogram shows quantitative representations of the levels of IL-1β-induced phosphorylation of STAT3 obtained from laser densitometric analysis of three independent experiments, respectively. Similar results were obtained with two additional and different cell preparations. *P < 0.05 in comparison to the value of IL-1β alone.