Co-localization of α-synuclein and ubiquitin in anterior horns of the lumbar (L4) spinal cord in hSOD1
G93A mice. Ubiquitin immunoreactivity (IR) is significantly reduced in the facial nucleus of the brainstem from melittin-treated hSOD1G93A mice (A, B). Scale bar = 200 μm (A, B). In the anterior horn of the spinal cord, the number of ubiquitin-expressing cells was increased in hSOD1G93A mice, but this was reduced by treatment with melittin (C, D). Scale bar = 100 μm (C, D). α-synuclein inclusions (E) were colocalized with ubiquitin (F) in motor neuron of the lumbar (L4) spinal cord in hSOD1G93A mice (G). Arrow head indicates double-labelled cells. Scale bar = 50 μm (E-G). MT: melittin, BS: brainstem, SP: spinal cord.