Figure 5

Co-localization of α-synuclein and ubiquitin in anterior horns of the lumbar (L4) spinal cord in hSOD1 ^G93A mice. Ubiquitin immunoreactivity (IR) is significantly reduced in the facial nucleus of the brainstem from melittin-treated hSOD1^G93A mice (A, B). Scale bar = 200 μm (A, B). In the anterior horn of the spinal cord, the number of ubiquitin-expressing cells was increased in hSOD1^G93A mice, but this was reduced by treatment with melittin (C, D). Scale bar = 100 μm (C, D). α-synuclein inclusions (E) were colocalized with ubiquitin (F) in motor neuron of the lumbar (L4) spinal cord in hSOD1^G93A mice (G). Arrow head indicates double-labelled cells. Scale bar = 50 μm (E-G). MT: melittin, BS: brainstem, SP: spinal cord.