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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Prevention of the β-amyloid peptide-induced inflammatory process by inhibition of double-stranded RNA-dependent protein kinase in primary murine mixed co-cultures

Figure 1

Cytotoxicity of compound C16 in neuron/astrocyte/microglia co-cultures. Scanning electron micrographs of neuron/astrocyte/microglia co-cultures prepared from brains of E18 C57Bl/6 mice. These co-cultures were incubated with the specific PKR inhibitor, C16 at 210 nM or 1 μM, or its DMSO vehicle in serum-free medium. The samples were examined in a JEOL JSM-840 electron microscope. Black or white arrows point out some microglia cells. Microglia appear as spherical smooth cells in contact with neurons and the astroglial layer in DMSO or 210 nM C16 conditions. However, in the presence of 1 μM C16, the integrity of cells is greatly altered with disruption of the axonal network, activated astrocytes with stellar form and microglia that look like macrophages. Scale bars: 10 μm, 15 μm and 20 μm for DMSO, 210 nM C16 and 1 μM C16, respectively.

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