Activation of IκB and NF-κB in lysates of murine astrocyte/neuron/microglia co-cultures. Representative blots showed the immunoreactivity of PS32/36-IκB (A), PS536-NF-κB (B) and corresponding total proteins in lysates of murine astrocyte/neuron/microglia co-cultures pretreated with 210 nM C16 and exposed to 20 μM Aβ42 for 72 h. Wells of blots correspond to DMSO, C16, Aβ42 and DMSO, Aβ42 and C16 from left to right. Results of phosphorylated proteins are reported relative to total protein. Results are expressed as a percentage compared to DMSO-treated cells (set at 100%). Results are mean ± SEM derived from 5 experiments in duplicate. #p < 0.05, ###p < 0.001 and *p < 0.05, ***p < 0.001 compared to respective controls without C16 by one-way ANOVA with Newman-Keuls multiple comparison test.